Figure 2. Excised pancreas during isolation and resulting islets. (A) Excised chronic pancreatitis pancreas cleaned of extraneous fat and tissue. The head, neck, body, and tail are labeled. (B) The pancreas head and body bisected at the neck with 14G catheters secured in the pancreatic ducts during perfusion with collagenase using a Rajotte controlled-temperature recirculation device. The head, body, and tail are labeled. Arrows are pointing to the bisected planes of the neck. (C) Sample of final islet cell mass of 30 to 40% purity. Islets are stained red with dithizone. Acinar tissue does not take up dithizone and is pale in color. Scale bar=100 µm.