22 December 2006
Apoptosis and proliferation of mesangial cells isolated from kidneys undergoing compensatory growth following contralateral nephrectomy: Role of the renin-angiotensin system
Joshua Weissgarten, Sylvia Berman, Shai Efrati, Micha Rapoport, Mirel Cohn, David Modai, Zhan AverbukhMed Sci Monit 2007; 13(1): BR16-23 :: ID: 470140
Abstract
Background: Contralateral nephrectomy stimulates compensatory growth of the remaining kidney. Intensive growth is frequently associated with increased apoptosis. The proliferation and apoptosis of cultured
rat mesangial cells isolated from the remaining kidney following contralateral nephrectomy were evaluated. The involvement of the renin-angiotensin system was concomitantly assessed.
Material/Methods: Apoptosis was assessed by TUNEL assay and hematoxylin staining, proliferation by 3H-thymidine incorporation, angiotensin-II (A-II) production by RIA, bradykinin synthesis by specifi c EIA, and AT-1/AT-2 receptor mRNA expression by RT-PCR. 125I-A-II labeling was applied for AT-1/AT-2 receptor density evaluation.
Results: Apoptosis of unstimulated control cultures progressively increased from a baseline of 2.02±0.55% to 8.3±1.19% in 30-min and 12.8±4.11% in 24-h cultures (p
Keywords: Renin-Angiotensin System - drug effects, Receptor, Angiotensin, Type 1 - metabolism, RNA, Messenger - metabolism, Nephrectomy, Mesangial Cells - drug effects, Gene Expression Regulation - drug effects, Angiotensin II Type 2 Receptor Blockers, Cell Separation, Cell Proliferation - drug effects, Captopril - pharmacology, Bradykinin - biosynthesis, Angiotensin I - metabolism, Angiotensin II - pharmacology
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
06 Mar 2024 : Clinical Research
Comparison of Outcomes between Single-Level and Double-Level Corpectomy in Thoracolumbar Reconstruction: A ...Med Sci Monit In Press; DOI: 10.12659/MSM.943797
21 Mar 2024 : Meta-Analysis
Economic Evaluation of COVID-19 Screening Tests and Surveillance Strategies in Low-Income, Middle-Income, a...Med Sci Monit In Press; DOI: 10.12659/MSM.943863
10 Apr 2024 : Clinical Research
Predicting Acute Cardiovascular Complications in COVID-19: Insights from a Specialized Cardiac Referral Dep...Med Sci Monit In Press; DOI: 10.12659/MSM.942612
06 Mar 2024 : Clinical Research
Enhanced Surgical Outcomes of Popliteal Cyst Excision: A Retrospective Study Comparing Arthroscopic Debride...Med Sci Monit In Press; DOI: 10.12659/MSM.941102
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952