08 February 2016 : Laboratory Research
Diethylstilbestrol Regulates the Expression of LGR8 in Mouse Gubernaculum Testis Cells
Shouxing DuanAEF, Xuewu JiangDG, Xuan ZhangDG, Lei XieBF, Zongbo SunBF, Shuhua MaCEG, Jianhong LiCEGDOI: 10.12659/MSM.895089
Med Sci Monit 2016; 22:416-421
Abstract
BACKGROUND: Hormonal effects on the gubernaculum can affect testicular descent. Diethylstilbestrol (DES) is a nonsteroidal synthetic estrogen that disrupts the outgrowth of gubernaculums, leading to testis maldescent. However, the underlying mechanisms remain elusive.
MATERIAL AND METHODS: The gubernaculum were removed from 3-day-old mice and cultured. The subcultured cells were randomly divided into a normal control group and experimental groups. The DES groups were administered 10 μg/ml, 1 μg/ml, 0.1 μg/ml, 0.01 μg/ml of diethylstilbestrol dissolved in dimethyl sulfoxide (DMSO) respectively. The cell morphology was observed under an inverted microscope, and leucine-rich repeat-containing G protein-coupled receptor 8 (LGR8) was localized by immunofluorescence. The expressions of LGR8 gene and protein in gubernaculum cells were quantified by RT-PCR and Flow Cytometer respectively.
RESULTS: DES treatment converted cells from a normal fibroblast-like morphology into a more refractile, spindle-shaped morphology or irregular elliptical shapes along with cytoplasmic shrinkage. LGR8 was expressed in the cytoplasmic membrane, DES dose-dependently downregulated LGR8 expression at low doses (≤1.0 μg/ml), but upregulated LGR8 at high doses (10 μg/ml) at both the mRNA and protein levels.
CONCLUSIONS: These results suggest that DES causes testicular maldescent by altering the LGR8 pathway in mouse gubernaculum testis cells.
Keywords: Cryptorchidism - metabolism, Diethylstilbestrol - pharmacology, Estrogens, Non-Steroidal - metabolism, Random Allocation, Receptors, G-Protein-Coupled - metabolism, Testis - metabolism
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