30 November 2010
Diet-induced obesity causes metabolic, endocrine and cardiac alterations in spontaneously hypertensive rats
Silvio A. Oliveira JrABCDEFG, Maeli Dal Pai-SilvaBD, Paula F. MartinezBDE, Ana P. Lima-LeopoldoB, Dijon H.S. CamposB, André S LeopoldoB, Marina P. OkoshiBDEF, Katashi OkoshiBDE, Carlos R. PadovaniACD, Antonio C. CicognaADEFGMed Sci Monit 2010; 16(12): BR367-373 :: ID: 881288
Abstract
Background: Although obesity has been associated with several effects in rodents, few investigations have evaluated the metabolic, endocrine, and cardiac parameters of spontaneously hypertensive rats (SHR) with dietary-induced obesity. The current study analyzed the influence of dietary-induced obesity on metabolic, endocrine, and cardiac characteristics in SHR.
Material/Methods: Male SHR were distributed in 2 groups: C-SHR (n=10) and OB-SHR (n=10). While C-SHR received a standard commercial diet (CD; 3.2 kcal/g), OB-SHR were submitted to a hypercaloric diet (HD; 4.6 kcal/g) for 20 weeks. Nutritional, metabolic, and endocrine evaluation involved measurement of calorie intake, dietary efficiency, body weight, adiposity, glycemia, triacylglycerol, insulin, and leptin. Cardiovascular evaluation integrated systolic blood pressure (SBP), echocardiography, gross and ultrastructural morphology, and myosin heavy chain (MHC) analyses of the myocardium.
Results: Animals in OB-SHR had greater values of BW, adiposity, triacylglycerol, and leptin and impaired glycemic tolerance compared with the C-SHR group. In the cardiovascular context, dietary-induced obesity increased interstitial collagen, the cardiomyocyte area, and the relative expression of beta-MHC, and well as beta-/alpha-isoform ratio of MHC. Likewise, OB-SHR showed ultrastructural morphologic alterations, with loss and disorganization of myofilaments, lipid droplets, severe mitochondrial damage, and T-tubule dilation. Concerning the in-vivo cardiovascular profile, although SBP and systolic function were unchanged by dietary-induced obesity, echocardiography results evidenced impaired diastolic function in OB-SHR in relation to their control counterparts.
Conclusions: Diet-induced obesity was associated with endocrine alterations, and it accentuated cardiac remodeling, promoting diastolic dysfunction of restrictive filling pattern in the SHR strain.
Keywords: Nutritional Status, Myosin Heavy Chains - analysis, Myocardium - ultrastructure, Leptin - blood, Insulin - blood, Energy Intake, Echocardiography, Diet - adverse effects, Body Weight, Blood Pressure, Adiposity, Obesity - pathology, Rats, Inbred SHR - physiology, Triglycerides - analysis
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
18 Apr 2024 : Clinical Research
Comparative Analysis of Open and Closed Sphincterotomy for the Treatment of Chronic Anal Fissure: Safety an...Med Sci Monit In Press; DOI: 10.12659/MSM.944127
08 Mar 2024 : Laboratory Research
Evaluation of Retentive Strength of 50 Endodontically-Treated Single-Rooted Mandibular Second Premolars Res...Med Sci Monit In Press; DOI: 10.12659/MSM.944110
11 Mar 2024 : Clinical Research
Comparison of Effects of Sugammadex and Neostigmine on Postoperative Neuromuscular Blockade Recovery in Pat...Med Sci Monit In Press; DOI: 10.12659/MSM.942773
12 Mar 2024 : Clinical Research
Comparing Neuromuscular Blockade Measurement Between Upper Arm (TOF Cuff®) and Eyelid (TOF Scan®) Using Miv...Med Sci Monit In Press; DOI: 10.12659/MSM.943630
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952