01 April 2004
Histopathological and immunohistochemical analysis of lymphoid follicles in labial salivary glands in primary and secondary Sjögren’s syndrome
Monika Prochorec-Sobieszek, Teresa Wagner, Marios Loukas, Hanna Chwalińska-Sadowska, Marzena OlesińskaMed Sci Monit 2004; 10(4): BR115-121 :: ID: 11618
Abstract
Background:Ectopic lymphoid follicles are considered morphological indices of local chronic antigen stimulation. The objective of this study was to present a detailed histopathological and immunohistochemical analysis of ectopic lymphoid follicles in salivary glands in Sjögren’s syndrome (SS), compared to lymphoid follicles in lymph nodes.
Material/Methods: Samples of labial salivary glands from 37 patients with primary and 57 with secondary SS were examined by histopathology and immunohistochemical methods with monoclonal antibodies (DAKO) to identify follicular dendritic cells, T and B lymphocytes, immunoglobulins and class II MHC HLA DPQR. Lymph nodes from 2 patients with primary SS were also examined for the same markers.
Results: Focal inflammatory infiltrations in labial salivary glands in 64.9% of primary and 23.1–49.6% secondary SS were found to morphologically mimic lymphoid follicles in various stages of development. Secondary ectopic lymphoid follicles resemble the histological structure and immunohistochemistry of germinal centers in lymphoid follicles in reactive lymph nodes due to the large number of centrally located B cells (CD20[sup]+[/sup]), reticular pattern of dendritic cells (CD21[sup]+[/sup]) and T helper/ inducer cells (CD4[sup]+[/sup]). The centers of secondary ectopic lymphoid follicles contain deposits of IgG and IgM.Conclusions: Ectopic lymphoid follicles quite often present in labial salivary gland in SS show structural and immunohistochemical features typical for lymphoid tissue. These ectopic follicles are the place of immune complex trapping, antigen presentation on dendritic cells and initiation of autoimmunologic response. This finding seems be very important for better understanding of the pathogenesis and diagnosis of SS as an autoimmunological disorder.
Keywords: Antigens, CD20 - immunology, Antigens, CD20 - metabolism, B-Lymphocytes - immunology, Dendritic Cells - immunology, HLA-DR Antigens - immunology, HLA-DR Antigens - metabolism, Immunoglobulins - immunology, Immunoglobulins - metabolism, Lymphoid Tissue - immunology, Lymphoid Tissue - pathology, Salivary Glands - immunology, Salivary Glands - pathology, Sjogren's Syndrome - immunology, Sjogren's Syndrome - pathology, T-Lymphocytes - immunology, Antigens, CD20 - metabolism, B-Lymphocytes - immunology, Dendritic Cells - immunology, HLA-DR Antigens - metabolism, Immunoglobulins - metabolism, Lymphoid Tissue - pathology, Salivary Glands - pathology, Sjogren's Syndrome - pathology, T-Lymphocytes - immunology
Editorial
01 May 2024 : Editorial
Editorial: First Regulatory Approval for Adoptive Cell Therapy with Autologous Tumor-Infiltrating Lymphocytes (TILs) – Lifileucel (Amtagvi)DOI: 10.12659/MSM.944927
Med Sci Monit 2024; 30:e944927
In Press
12 Mar 2024 : Clinical Research
Metabolomic Alterations in Methotrexate Treatment of Moderate-to-Severe PsoriasisMed Sci Monit In Press; DOI: 10.12659/MSM.943360
14 Mar 2024 : Clinical Research
Renal Dysfunction Increases Risk of Adverse Cardiovascular Events in 5-Year Follow-Up Study of Intermediate...Med Sci Monit In Press; DOI: 10.12659/MSM.943956
15 Mar 2024 : Clinical Research
Impact of One-Lung Ventilation on Oxygenation and Ventilation Time in Thoracoscopic Heart Surgery: A Compar...Med Sci Monit In Press; DOI: 10.12659/MSM.943089
14 Mar 2024 : Clinical Research
Differential DHA and EPA Levels in Women with Preterm and Term Births: A Tertiary Hospital Study in IndonesiaMed Sci Monit In Press; DOI: 10.12659/MSM.943895
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952